EMortal™ is a hydrolyzed pea protein, a peptone. Actually EMortal™ is a small peptide mixture extracted from Pea( Pisum sativum L.). The peptides are extracted through a process that uses no harmful chemicals. The activity of EMortal™ on the stem cells has been proven by scientific research and a clinical trial.
Since the peptides have no antigens, they are not recognized as foreign by the host body because of their size and there are no allergic reactions. The use of plant origin to stimulate the stem cells also eliminates ethical considerations, since it causes no harm to the animal or involvement with human tissues.
Cell culture technology is used in virtually all fields of biomedical research and testing. We have developed a stem cell culture model for finding new compounds to activate stem cell proliferation. The effects of TGF-β1- elevating agents on the activity of TGF-β1 promoter are studied using the luciferase(LUC)-reporter-gene system.
The cytokine TGF-β1 plays an important role in regulating the wound healing and tissue repair process; and aberration of its action has been implicated in several pathological processes including cancer, autoimmune disease and fibrosis. Specifically in the skin, TGF-β1 induces proliferation of dermal fibroblasts and generates increased levels of Hyaluronan(HA), matrix polysaccharide. In the skin, the extracellular matrix(ECM) is composed of HA and other sulfated GAGs, combined with collagen and elastin. Large amounts of water are held in the ECM. When elastin is not bathed in water, it becomes dry and brittle, thus the look of dry, brittle, wrinkled skin. HA also influence fibroblast proliferation and thereby affect wound healing outcomes.
In skin keratinocytes, TGF-β1 has been shown to inhibit growth and to rapidly reduce protooncogene, c-myc expression. TGF-β1 is a potent tumor suppressor.
Therefore TGF-β1 activators are very useful for skin care products and potentially become stem cell activators.
Using this culture model we have found a few TGF-β1 activators including EMortal™. Stimulation of the transfected stem cells with EMortal™ enhanced both TGF-β1 dependent transcription of luciferase gene and cell growth.